Bt2a bt2b primers
WebFeb 15, 2024 · 表1 用于苹果褐腐病鉴定的引物信息Table 1 Primers used in this study 取5 μL上述 PCR 扩增产物于1.2%琼脂糖凝胶电泳(U：110 V)进行检测后将 PCR 产物送至上海生工生物工程有限公司进行测序。 WebDescription. Both B2M (Beta-2-Microglobulin) and CIITA (Class II Transactivator) have been genetically removed from THP-1 cells using CRISPR/Cas9 genome editing. Purchase of …
Bt2a bt2b primers
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WebJan 11, 2024 · To confirm the morphological identification, the internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α) and β-tubulin genes of the representative isolate Wn-4 were sequenced using the primers ITS1/4, EF1-728 F/986R and Bt2a/Bt2b. WebMay 27, 2014 · This protocol could be completed in approximately 30 min and utilised a combination of physical and chemical extraction methods but did not require organic solvents nor ethanol precipitation. The method reduces the number of tube manipulations and yielded suitable template DNA for PCR amplification from all phyla that were tested. …
WebJun 7, 2024 · The Beta-tubulin-1 gene region ( BT 1) was amplified using primers Bt1A and Bt1B (Glass and Donaldson 1995 ), the RNA … WebMay 16, 2024 · The ITS, EF1-α and tub2 gene regions of the reference isolate (Code: WL2-D) were sequenced using primers ITS1/ITS4, EF1-728F/EF1-986R and BT2a/BT2b, and the sequences were deposited in GenBank with accession numbers MZ028464 (ITS), MW862012 (TEF1-α) and MZ028463 (tub2).
WebThe electrophoretogram showing the behaviour of primer pair Bt2a and Bt2b in a temperature gradient (annealing 50–60 °C). The specificity for the tubC paralogue … WebSep 1, 2024 · Bt2 primers can amplify either benA fragments (three introns) or paralogous tubC fragments (two introns) in the Nigri section. When a mix of those two paralogues is used in phylogenies, this can create incongruent …
Web黄宏强，范小燕，陶亚群，鲁红学，邓建新 (长江大学农学院，湖北，荆州 434025) 三七黑斑病病原菌的复核鉴定
WebNucleic acid sequence analysis is an extensively applied method in Genomics studies. Universal primers are complementary to nucleotide sequences that are very common in a particular set of DNA molecules and cloning vectors. Thus, they are able to bind to a wide variety of DNA templates. During sequencing, primers are annealed to the denatured ... sample of natural disasterWebUsing the primer setBt2aandBt2b,a362-bpfragmentwasamplifiedfromP.striiformis f. sp. tritici, while a 496-bp fragment was amplified from both P.triticinaandP.graminisf.sp.tritici (Fig.1).TheDNAfragments of P. triticina and P. graminis f. sp. tritici shared 100% homology, whereas the specific DNAfragment of P. striiformis f.sp.tritici shared … sample of national geographic for kidsWebSep 18, 2005 · Here we report the first high-resolution structure of any portion of the telomerase reverse transcriptase, the telomerase essential N-terminal (TEN) domain … sample of narrative report in ojtWebInterchangeable Core Lock Pin, Best, Top, #2, 0.108 Diameter x 0.025 Length, Brass, For A2 System, 100 each per Bag sample of ncnda agreementWebNov 22, 2011 · Sequence analysis of the partial β-tubulin ( benA) and partial calmodulin ( CaM) genes were performed using the Bt2a and Bt2b (Glass and Donaldson 1995) and CL1 and CL2A primers (O’Donnell et al. 2000 ). Phylogenetic analysis was performed using the two loci that were analyzed together with 24 representative strains of section Nigri … sample of nature of workWebMay 13, 2015 · 3 Present address: Institute for Cell Engineering, Departments of Neurology and Neuroscience, Johns Hopkins University School of Medicine, 733 North Broadway, … sample of new zealand permanent resident cardWebWe developed and validated a discriminative low-cost PCR-based test to discriminate Aspergillus isolates at the species level. The Beta tubulin gene of various reference … sample of negative feedback