Nisin a promoter
Webb1 jan. 1999 · Abstract. Nisin biosynthesis is autoregulated extracellularly by the mature and modified peptide. To investigate other regulatory effects on nisin biosynthesis, a … http://www.macklin.cn/article/detail/339
Nisin a promoter
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Webb31 juli 1990 · The nisR promoter was shown to direct nisin-independent gusA expression in L. lactis MG 1363, which is a nisin-transposon- and plasmid-free strain. L. lactis NZ9800, which does not produce nisin because of a deletion in the nisA gene, containing the nisF-gusA fusion plasmid, gave rise to beta-glucuronidase production only after … Webb17 jan. 2024 · In this study, we obtained three spontaneous mutants that were highly resistant to nisin A, designated as SAN (Staphylococcus aureus nisin resistant) 1, SAN8, and SAN87.We identified that SAN1 had a single mutation in the promoter region of braRS which is a two‐component system, while SAN8 and SAN87 had single mutations …
Webb28 juni 2008 · A DNA fragment containing the nisA gene and its native P nis promoter was amplified, and cloned into pPTPL (a reporter vector with a promoterless lacZ) to generate pDF03. This was subsequently introduced into L. lactis NZ9800. NZ9800 is derivative of a nisin-producing strain, L. lactis NZ9700, from which the nisA gene has been deleted . WebbThe biosynthesis of nisin is regulated in a growth-phase-dependent manner including nisin-mediated induction which occurs via NisRK two-component regulatory system. This review outlines some of the more recent developments ... ArticleTitle Functional analysis of promoters in the nisin gene cluster of Lactococcus lactis J. Bacteriol. 178 3434 ...
Webb29 mars 2024 · Nisin is a natural antimicrobial peptide that can be conjugated with silver nanoparticles to enhance its antibacterial properties. The concentration of the conjugate can be optimized based on the... WebbNisin, a typical lantibiotic, has robust antimicrobial activity combined with limited cytotoxicity, and the development of resistance to it is slow. These properties make …
Webb8 juli 2015 · Nisin is a small, heat-stable and biochemically well-characterized natural antibacterial peptide (Mulders et al. 1991) and it could be used as a convenient reporter …
Webb17 jan. 2024 · Nisin is an antimicrobial peptide whose biosynthesis is controlled by a cluster of 11 genes. Among them, nisR and nisK encode transmembrane receptor NisK … frost and sullivan revenueWebbNational Center for Biotechnology Information frost and sullivan researchWebbAbstract The nisA promoter is positively regulated in Lactococcus lactis ATCC 11454 by autoinduction via a two-component NisRK-mediated system. However, induction of this … ghr leapWebb10 nov. 1995 · In further experiments, the nisin-producing strain NZ9700 with either plasmid pNZ273 (containing the promoterless gusA gene) or pNZ8008 (containing the nisA promoter fragment followed by the gusA gene) was used in an agar diffusion assay (8) to determine the amount of nisin produced.Fifty times lower nisin production and … frost and sullivan scamWebb20 jan. 2013 · Nisin is a food grade inducer and can induce gene expression in a dynamic range of 10,000-folds [ 12 ]. However, the steep induction curve for expression level … frost and sullivan reviewsWebbA set of shuttle vectors, able to replicate in Escherichia coli and in gram-positive bacteria, containing a nisin-inducible promoter (PnisA) and genes encoding NisR and NisK, the … ghr law firm salemWebb1 jan. 2001 · Nisin is a small, 34-amino acid, cationic, hydrophobic peptide that belongs to the lantibiotic class of bacteriocins because of the presence of unusual amino acids such as lanthionine and β-methyllanthionine in the mature peptide. ghr leadership