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8.0 × 105 A549 cells were seeded to 10 cm2dish, after incubating for 6 h, the TMPyP4 or TPyP4-Pt was added to medium (a final concentration of 0.5 μM). After incubating for 2 days, cells were harvested and total RNA were isolated by Trizol (Takara) extraction. 3 μg RNA per sample was used as input material for the … Visa mer A549, HeLa, U2OS and SAOS2 were obtained from Cell Resource Center of Peking Union Medical College and cultured at 37 °C under 5% CO2in DMEM (gibco) supplemented with … Visa mer Long-term cell proliferation experiments were carried out using A549, HeLa, U2OS and SAOS2 cancer cell lines. 5.0 × 105 cells were seeded to … Visa mer The 96-well plate was coated with 2.5 μg/ml human fibronectin in PBS (Millipore, CA) for 2 h at room temperature. Cells were seeded into the … Visa mer Cells were seeded in 10 cm2 dish at a density of 5.0 × 105cells per dish and incubated at 37 °C for ~6 h until cells attached to the dish. TMPyP4 with final concentration of 1.0 or … Visa mer WebbKadi-Liis is showing you how to properly change a motocross/enduro tire. She has a new TMT4 Lite machine in her hands for that. See more at www.tiremountingt... browning vtws-216
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